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Ignarro's spectral analysis |
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| Louis Ignarro
reported that EDRF relaxed blood vessels. He also
identified EDRF as a molecule by using spectral
analysis of hemoglobin. When hemoglobin was exposed to
EDRF, maximum absorbance moved to a new wave-length;
and exposed to NO, exactly the same shift in absorbance
occurred! EDRF was identical with NO. A new principle
for signalling between human cells was
discovered. Spectrophotometry |
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Hemoglobin (yellow) exposed to endothelial cells that were stimulated to produce EDRF (green) |
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Hemoglobin (yellow) directly exposed to NO (green) The shift of absorption curves is identical, hence EDRF is NO |