The Nobel Prize in Chemistry 2006
Roger D. Kornberg
My adult scientific career began with graduate study in chemical physics with Harden McConnell at Stanford. I had the idea of elucidating the mechanism of ion transport across biological membranes by nuclear resonance. I thought ion transport must involve rotation of the transport protein in the membrane. Struggling to prove this wrong idea, it occurred to me to study the rotation in the membrane of a lipid molecule, about 1,000 molecular weight, rather than a protein fifty times larger. This led to my discoveries, by nuclear and paramagnetic resonance methods, of phospholipid flip-flop, an exceedingly slow process, and lateral diffusion, exceedingly fast (Kornberg and McConnell, 1971a ; Kornberg and McConnell, 1971b).
For postdoctoral work, I wanted to learn about the other important method of physico-chemical analysis of macromolecules, X-ray diffraction. The obvious choice was the Laboratory of Molecular Biology (LMB) in Cambridge, where protein crystallography was developed and still most intensively practiced at the time. I went in the spring of 1972 to work with Aaron Klug, who was not only a leading crystallographer, but also responsible for the application of Fourier methods to electron microscopy and image processing. While looking for a problem to study by X-ray diffraction, I got to know Mark Bretscher, the only person at the LMB interested in membrane structure, and he suggested reading a paper just published by Francis Crick titled "A General Model for Higher Organism Chromosomes" (Crick, 1971). Figure 3 of that paper was a diagram showing a loop of DNA crossed by a dashed line, said to symbolize a histone molecule. When I raised the subject with Aaron Klug, he immediately produced a sheaf of papers on the X-ray analysis of chromosomal material, or "chromatin," known for nearly a century to contain roughly equal weights of histones and DNA. Aaron had discussed the interpretation of the X-ray pattern of chromatin extensively with Francis, and he encouraged me to pursue the problem. He warned me, however, that it was a "messy" problem.
Notorious might have been a better word. Many had succumbed to the allure of the problem, with its potential for insight into genetic chemistry, only to be frustrated by the intractability of the histones. These proteins were, on the one hand, surprisingly simple, and on the other hand, hopelessly complicated. There are only five types of histone, designated H1, H2A, H2B, H3, and H4. Upon isolation, however, the individual histones proved to be extraordinarily sticky, binding avidly to DNA and interacting with one another in every possible combination. Whereas the X-ray diffraction pattern of chromatin was indicative of repeating order, the biochemical behavior of the histones did not appear to explain it. There was, moreover, sufficient variation in the relative amounts of the histone types in various tissues and organisms "to make the idea of a unique repeating order untenable" (Huberman, 1973). The histones came to be regarded as a kind of amorphous glue, coating the chromosomal DNA, with no obvious significance.
I began by repeating the work of others, isolating the individual histones, mixing them in various combinations with DNA, and recording X-ray diffraction patterns. I also scoured the literature and came across two papers that influenced my thinking. A paper by Hewish and Burgoyne reported the cleavage of about 10% of chromosomal DNA by an endogenous nuclease in isolated rat liver nuclei to multiples of a unit size (Hewish and Burgoyne, 1973). When I mentioned this to Francis Crick, he shot off a letter to Hewish inquiring about the size. The reply came from Burgoyne, giving the sedimentation coefficient of the unit length of DNA. Assuming the measurement was made under alkaline conditions, as was customary for sedimentation analysis of RNA, much studied at the LMB at the time, the value from Burgoyne corresponded to about 500 bases. This unit size did not relate to any other information about chromatin, and the appearance of multiples of the unit size simply confirmed what we already knew from X-ray diffraction, that chromatin contained some amount of repeating substructure.
The second paper, by van der Westhuyzen and von Holt, reported the extraction of histones from chromatin by mild methods, rather than with strong acid or other harsh treatment, as was customary at the time (van der Westhuyzen and von Holt, 1971). Mild methods failed to resolve the histones entirely from one another, so the paper was ignored. What attracted my attention was the clean separation of the mildly extracted histones into two groups, H2A/H2B, and H3/H4. This separation contrasted with the promiscuous interactions of the histones previously observed. I realized this promiscuity was likely attributable to the denaturation of histones during isolation in the past. From the data in the paper, I could also deduce that the H3/H4 group behaved as if twice the size of the H2A/H2B group, although all four individual histone proteins were about the same size. I concluded that H3 and H4 must form a dimer, and I thought I might crystallize and solve the structure of this unique histone oligomer.
What followed was truly astounding. I measured the molecular weight of the purified H3/H4 preparation by equilibrium ultracentrifugation, while Jean Thomas offered to analyze the material by chemical cross-linking. Both methods showed unequivocally that H3 and H4 were in the form of a double dimer, an (H3)2(H4)2 tetramer (Kornberg and Thomas, 1974). I pondered this result for days, and came to the following conclusions (Kornberg, 1974). First, the exact equivalence of H3 and H4 in the tetramer implied that the differences in relative amounts of the histones from various sources measured in the past must be due to experimental error. This and the stoichiometry of the tetramer implied a unit of structure in chromatin based on two each of the four histones, or an (H2A)2(H2B)2(H3)2(H4)2 octamer. Second, since chromatin from all sources contains roughly one of each histone for every 100 bp of DNA, a histone octamer would be associated with 200 bp of DNA. Finally, the (H3)2(H4)2 tetramer was reminiscent of hemoglobin, an a2b2 tetramer. The X-ray structures of hemoglobin and other oligomeric proteins available at the time were compact, with no holes through which a molecule the size of DNA might pass. Rather, the DNA in chromatin must be wrapped on the outside of the histone octamer.
As I turned these ideas over in mind, it struck me how I might explain the results of Hewish and Burgoyne. What if their sedimentation coefficient of unit length DNA fragments was measured under neutral rather than alkaline conditions? Then the DNA would have been double stranded and about 250 bp in length. Allowing for the approximate nature of the result, the correspondence with my prediction of 200 bp was electrifying. Then I recalled a reference near the end of the Hewish and Burgoyne paper to a report of a similar pattern of DNA fragments by Williamson. I rushed to the library and found that Williamson had obtained a ladder of DNA fragments from the cytoplasm of necrotic cells and measured the unit size by sedimentation under neutral conditions: the result was 205 bp! I was euphoric. In the months and years to follow, it was often pointed out how thin was the support for my ideas and how extended the line of reasoning, but I never really doubted the conclusions. The prediction of the DNA unit size and its verification convinced me completely.
Support for a particulate substructure of chromatin came from electron microscopy and from nuclease digestion and sedimentation analysis. Some work on these lines was done even before my own, and though not definitive, was nicely coincident with my ideas. In the years to follow, with colleagues in Cambridge, I proved the existence of the histone octamer and the equivalence of the 200 bp unit with the particle seen in the electron microscope (Kornberg, 1977). This chapter of the chromatin story concluded with the X-ray crystal structure determination of the particle, now known as the nucleosome, showing a histone octamer surrounded by DNA, in near atomic detail (Luger et al., 1997).
At this natural break in the work, I returned to the US, first as faculty member of the Department of Biological Chemistry of Harvard Medical School in 1976, and then the Department of Structural Biology of Stanford Medical School in 1978. I had decided to pursue the function rather than the structure of the nucleosome, and was joined in this by Yahli Lorch, who became my lifelong partner in chromatin research, and also my partner in life. We investigated the consequences of the nucleosome for transcription. It was believed that histones are generally inhibitory to transcription. We found, to the contrary, that RNA polymerases are capable of reading right through a nucleosome. Coiling of promoter DNA in a nucleosome, however, abolished initiation by RNA polymerase II (pol II) (Lorch et al., 1987). This finding, together with genetic studies of Michael Grunstein and colleagues, identified a regulatory role of the nucleosome in transcription. It has since emerged that nucleosomes play regulatory roles in a wide range of chromosomal transactions. A whole new field has emerged, one of the most active in bioscience today. It involves a bewildering variety of posttranslational modifications of the histones, and a protein machinery of great complexity for applying, recognizing, and removing these modifications.
Although Yahli's first priority was the rearing of our children, Guy, Maya, and Gil, she performed a series of important studies of chromatin remodeling, believed to oppose inhibition by nucleosomes. She began with Brad Cairns, who discovered the RSC chromatin remodeling complex. Subsequent work showed that RSC disrupts nucleosome structure and slides histone octamers along the DNA (Lorch et al., 1998; Lorch et al., 2001). Most recently, we found that RSC can transfer a histone octamer to a histone chaperone protein, exposing the nucleosomal DNA for transactions such as transcription (Lorch et al., 2006).
For studies of transcriptional regulation, it was necessary to identify the transcription machinery. Biochemical work, in the laboratories of Robert Roeder, Ronald and Joan Conaway, and others, revealed the complexity of the RNA polymerase II transcription machinery of mammalian cells, and it was apparent there was much to be gained by studies in yeast. A combined genetic and biochemical approach, possible only in yeast, would be advantageous for the solution of such a difficult problem. There was, however, a major obstacle. The development of a yeast pol II transcription system had been attempted unsuccessfully in many laboratories for more than a decade. Efforts to develop biochemical systems from yeast for RNA splicing, membrane transport, and other processes had also met with failure, leading to the widely held view that yeast, although the organism of choice for eukaryotic genetic analysis, was unsuited to biochemical investigation. First Yahli, and then graduate student Neal Lue, took up the challenge. Neal was eventually successful, by changing a number of procedures and conditions used in the past, including the replacement of chloride by acetate ion (Lue and Kornberg, 1987). Remarkably, chloride, though a component of other transcription systems, is highly inhibitory to yeast pol II transcription.
Neal, Ray Kelleher, and Peter Flanagan undertook fractionation of the yeast system, followed by Mike Sayre, John Feaver, Herbert Tschochner, Opher Gileadi, and Lynn Henry. The yeast system was problematic, since the starting transcription signal was a thousand-fold less than that in mammalian systems, and we soon reached an impasse. After enriching the transcription proteins about a hundred-fold through two chromatographic steps, we could proceed no further. Every subsequent step eliminated transcription completely. Mike achieved a breakthrough by starting over. He realized the problem was the loss of essential components and an accumulation of inhibitors, and he devised a new fractionation procedure that was ultimately successful (Sayre et al., 1992).
An important outcome of this work was the discovery that yeast and mammalian pol II systems are the same. Differences in promoter structure believed to reflect fundamental differences in the transcription proteins proved to be insignificant. The real payoff, however, was in the study of transcriptional regulation. In the late 1980s, it was thought the communication between gene activator proteins and the transcription machinery was direct. In 1990 and 1991, Ray and Peter produced evidence for an additional factor required for this communication in a still crude yeast system (Flanagan et al., 1991; Kelleher et al., 1990). This additional factor, which we referred to as Mediator, proved elusive. The requirement for the factor was variable over the course of fractionation. As so often happens, the solution of the problem came from an unexpected direction. The most difficult protein of the pol II transcription system to purify, known as factor IIH, was contaminated with Mediator, and only after entirely pure IIH was obtained by Jesper Svejstrup (Svejstrup et al., 1995) were Stefan Björklund and Young-Joon Kim then able to complete the isolation of Mediator (Kim et al., 1994). To our astonishment, pure Mediator proved to be an assembly of nearly two dozen proteins, more than half of which were known from genetic studies in yeast to be involved in transcriptional regulation.
In parallel with these biochemical studies of pol II transcription, we pursued the structure of the transcription machinery. Pol II, the central component of the machinery, is an assembly of a dozen proteins, several times the size of any asymmetric structure determined by X-ray diffraction in the early 1980s when we began. The story of our solution of the pol II problem is told in my Nobel Lecture, except for its origins in my graduate studies of lipid diffusion. While I was in Cambridge, Aaron Klug and collaborators were developing electron microscope crystallography for the solution of protein structures too large for X-ray crystallography. As the name implies, the electron microscope approach also requires protein crystals, only they must be thin, preferably a single protein molecule thick, or "two-dimensional," for transmission of the electron beam. The work in Cambridge at the time was limited to naturally occurring ordered arrays of proteins, and it was apparent that a general method of forming two-dimensional crystals was needed to bring any protein of interest within reach of the electron microscope procedure. I thought I might exploit lateral diffusion in lipid layers for the purpose. A protein bound to a lipid layer would be constrained in two dimensions but free to diffuse and, I hoped, to crystallize. I tried this idea, beginning with nucleosomes and positively charged lipid layers. Finally, after repeated failures over a period of about five years, a sabbatical visitor, Ed Uzgiris, was successful, with the combination of a monoclonal antibody directed against a lipid hapten (Uzgiris and Kornberg, 1983). This work led eventually to twodimensional crystals of pol II, and then to large single crystals and the X-ray structure of pol II.
Our biochemical and crystallographic work converged in the X-ray structure determination of pol II. This convergence was the culmination of a long effort, but also a beginning. Pol II associates with two dozen additional transcription proteins and with Mediator in a giant assembly formed at every promoter prior to the initiation of transcription. The ultimate goal of our work is to solve this giant complex and thus to understand the mechanism and regulation of transcription. We believe we will achieve this goal within the next decade, through crystallography and, possibly, another development in electron microscopy. Inspired by graduate student Grant Jensen, we have pursued the synthesis of large heavy atom clusters, for the purpose of structure determination without crystals at near atomic resolution (Jensen and Kornberg, 1998). This work has opened a window on a whole new realm of inorganic chemistry and materials science, through which we may pass into the future.
|Crick, F. (1971). General model for the chromosomes of higher organisms. Nature 234, 25-27.|
|Flanagan, P. M., Kelleher III, R. J., Sayre, M. H., Tschochner, H., and Kornberg, R. D. (1991). A mediator for activation of RNA polymerase II transcription in vitro. Nature 350, 436-438.|
|Hewish, D. R., and Burgoyne, L. A. (1973). Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochem. Biophys. Res. Commun. 52, 504-510.|
|Huberman, J. A. (1973). Structure of chromosome fibers and chromosomes. Annu. Rev. Biochem. 42, 355-378.|
|Jensen, G. J., and Kornberg, R. D. (1998). Single-particle selection and alignment with heavy atom cluster-antibody conjugates. Proc. Natl. Acad. Sci. USA 95, 9262-9267.|
|Kelleher, I., R.J., Flanagan, P. M., and Kornberg, R. D. (1990). A novel mediator between activator proteins and the RNA polymerase II transcription apparatus. Cell 61, 1209- 1215.|
|Kim, Y. J., Björklund, S., Li, Y., Sayre, M. H., and Kornberg, R. D. (1994). A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. Cell 77, 599-608.|
|Kornberg, R. D. (1974). Chromatin structure: a repeating unit of histones and DNA. Science 184, 868-871.|
|Kornberg, R. D. (1977). Structure of chromatin. Ann. Rev. Biochem. 46, 931-954.|
|Kornberg, R. D., and McConnell, H. M. (1971a). Inside-outside transitions of phospholipids in vesicle membranes. Biochemistry 10, 1111-1120.|
|Kornberg, R. D., and McConnell, H. M. (1971b). Lateral diffusion of phospholipids in a vesicle membrane. Proc. Natl. Acad. Sci. USA 68, 2564-2568.|
|Kornberg, R. D., and Thomas, J. O. (1974). Chromatin structure; oligomers of the histones. Science 184, 865-868.|
|Lorch, Y., Cairns, B. R., Zhang, M., and Kornberg, R. D. (1998). Activated RSC-nucleosome complex and persistently altered form of the nucleosome. Cell 94, 29-34.|
|Lorch, Y., LaPointe, J. W., and Kornberg, R. D. (1987). Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones. Cell 49, 203-210.|
|Lorch, Y., Maier-Davis, B., and Kornberg, R. D. (2006). Chromatin remodeling by nucleosome disassembly in vitro. Proc. Natl. Acad. Sci. USA 103, 3090-3093.|
|Lorch, Y., Zhang, M., and Kornberg, R. D. (2001). RSC unravels the nucleosome. Mol. Cell 7, 89-95.|
|Lue, N. F., and Kornberg, R. D. (1987). Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 84, 8839-8843.|
|Luger, K., Mader, A. W., Richmond, R. K., Sargent, D. F., and Richmond, T. J. (1997). Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature 389, 251-260.|
|Sayre, M. H., Tschochner, H., and Kornberg, R. D. (1992). Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J. Biol. Chem. 267, 23376-23382.|
|Svejstrup, J. Q., Wang, Z., Feaver, W. J., Wu, X., Bushnell, D. A., Donahue, T. F., Friedberg, E. C., and Kornberg, R. D. (1995). Different forms of TFIIH for transcription and DNA repair: Holo-TFIIH and a nucleotide excision repairosome. Cell 80, 21-28.|
|Uzgiris, E. E., and Kornberg, R. D. (1983). Two-dimensional crystallization technique for imaging macromolecules, with an application to antigen-antibody-complement complexes. Nature 301, 125-129.|
|van der Westhuyzen, D. R., and von Holt, C. (1971). A new procedure for the isolation and fractionation of histones. FEBS Letters 14, 333-337.|
From Les Prix Nobel. The Nobel Prizes 2006, Editor Karl Grandin, [Nobel Foundation], Stockholm, 2007
This autobiography/biography was written at the time of the award and first published in the book series Les Prix Nobel. It was later edited and republished in Nobel Lectures. To cite this document, always state the source as shown above.Copyright © The Nobel Foundation 2006